The Code Breaker
by John Lawrence, March26, 2021
The Code Breaker by Walter Isaacson is an exciting new book which details the efforts and the knowledge to edit genes known as CRISPR. That means cutting into the DNA genome and removing "sick" genes and/or replacing them with the correct genes. The book details the quest to discover the mechanism by which this is possible. The book focuses on Jennifer Doudna but many researchers, many of them recent immigrants or workers at foreign universities, are involved. The fundamental insights can be explained in a few sentences. They noticed that bacteria have been fighting off viruses successfully for billions of years, and they were doing this by incorporating the virus DNA into the data base of their own genome. Upon encountering a new virus the bacteria knew how to encode that virus's DNA so that they could identify it in an attack in the future. Secondly, the bacteria knew how to dispatch a portion of RNA that would travel to the site of the virus and encode an enzyme that would then destroy the virus. The messenger RNA knew precisely where to point the enzyme which then cut the virus DNA thereby destroying it. So the e. coli bacterium has known how to edit genes for billions of years. The trick is to figure out exactly how the bacterium does this.
So Jennifer Doudna and cohorts did just that: They figured out how a bacterium cuts a virus's DNA precisely in a certain location. That's what the book is about. That's what gene editing is about. Gene editing is in its infancy, but it has already been used to correct the genetic mutation which causes sickle cell anemia which is caused by the mutation in one gene. Experimentally, it has been shown that if this gene can be cut out and replaced by one that contains the correct genetic information, the disease can be cured. If this can be done early in the development of an embryo, the correct gene can even be transmitted to succeeding generations. An estimated 8 million children are born each year with severe genetic disorders or birth defects. Gene editing can possibly correct some or all of these defects. In order to do this the enzyme which cuts the gene out of the DNA must be told precisely where to cut, and the correct gene must somehow be implanted precisely at this location and the DNA reconnected. In theory the whole idea is very simple. Figuring out how to do this either in the laboratory or in actual animals, much less in humans, is the hard part.
Whether or not this research will lead to an understanding of the origins of life is an open question. Perhaps there is a sub-atomic entity sort of like a neutrino which is massless and ubiquitous in the universe which is the progenitor of life wherever conditions are propitious. Perhaps it is able to generate an organism with the minimal genome, something which has never been discovered in nature. Even a virus which has the simplest known genomic structure has three thousand base pairs, compared to the three billion base pairs in human genomes. A base pair consists of the 4 nucleotides called A, C, G, T which comprise one rung of the DNA ladder. So a successful gene edit would have to identify the precise rung of the DNA ladder where the mutation had occurred which means at least one of the 4 letters of the base pair was incorrect and correct it.
So I theorize that a life progenitor would have to be something that is ubiquitous in the universe and capable of starting life with a minimal genome which consists of only a few base pairs. This is a mystery but certainly not the only one. Gravity is an every day phenomenon but no one has detected a graviton which is thought to be the physical entity that distorts space time according to Einstein's theory of relativity. No one understands the "dark energy" which is thought to be responsible for the fact that the universe is not only expanding but accelerating. No one understands what Einstein called "spooky action at a distance" which has been demonstrated to be a reality in quantum theory. Just as the line is blurred between what is material and what is massless energy, the line is blurred between what is spiritual and what is material. Is the creation of life in the universe a physical, material phenomenon or a spiritual one?
